You do not need to upgrade from version 10.0.0 to version 10.0.1.
Before using a version 10 MobiLink server, check the behavior changes to see if any affect you. See SQL Anywhere® 10 - Changes and Upgrading.
Version 10 of the MobiLink server only supports version 8 and 9 SQL Anywhere and UltraLite clients. If you need to support earlier clients, you should keep an earlier version of the MobiLink server for supporting them.